Characterization of intervertebral disc degeneration by high
(TOCSY)) 1H HR-MAS spectroscopy, and T1 and T2 relaxation time measurements to ... Key words: intervertebral disc degeneration; proton HR-MAS spectroscopy; chondroitin .... model of human tissue degradation. However, the differ-.
Characterization of metabolites determined by means of 1H HR
Aug 10, 2014 ... After standard preparation of the operation field, intervertebral disc exposure was .... Metabolites assigned in HR MAS spectra of disc tissues, their ..... proton NMR analysis of human prostate tissue with slow spinning rates.
2H double quantum filtered (DQF) NMR spectroscopy of the nucleus
the Nucleus Pulposus Tissues of the Intervertebral Disc. William Perea,1 ... copy of nucleus pulposus (NP) tissues from human interverte- bral discs is reported. .... using HR-MAS spectroscopy and correlated with degener- ative grade (20).
In vivo quantification of human lumbar disc degeneration using T1ρ
Keywords. Intervertebral disc degenerationMagnetic resonance imaging (MRI) Spin .... The severely degenerate L5S1 disc was not included in analysis due to lack of tissue ..... by high-resolution magic angle spinning (HR-MAS) spectroscopy.
Jeffrey C. Lotz, Ph.D. - Applied BioMechanics
Member, Musculoskeletal Tissue Engineering Study Section, NIH, 2005-2009 ... Cheng, K.K., Berven, S.H., Hu, S.S., and Lotz, J.C., Intervertebral discs from spinal ... Morphology of the human vertebral endplate, The Journal of Orthopaedic ..... J.C., Kurhanewicz, J., and Majumdar, S., Correlation of HR-MAS spectroscopy.
Full-Text PDF - MDPI.com
Oct 31, 2016 ... for investigating metabolic profiles from ex vivo tissue samples [7–9]. ... applied to the study of articular cartilage degradation , intervertebral disc degeneration [ 21], ... analysis of bone tumor lesions in human patients. Here ... 1H HR-MAS spectra were obtained for seven muscle (group A: A1–A3, A5–A8), ...
Transplantation of human Wharton's jelly-derived mesenchymal
Oct 2, 2015 ... Adipose tissue-derived mesenchymal stem cell ... Intervertebral disc (IVD) degeneration is considered to be a major cause of low back ...... disc degeneration by high-resolution magic angle spinning (HR-MAS) spectroscopy.
Separation and Quantification of Lactate and Lipid at 1.3 ppm by
aration of lactate and lipid in tumor tissue (22,23). Spec- tral editing methods such as ... (37–39) and proteoglycans (PGs) in intervertebral discs. (40).
The mechanism of aggrecan release from cartilage differs - NCBI
The mechanisms of aggrecan degradation in adult human ar- ticular, adult ... charge density provides the tissue with its osmotic properties. ..... mass bands reactive with the anti-AGG-C antibody were seen in .... degradation in human intervertebral disc and articular cartilage. .... spectroscopy using spin trapping reagents.
Plasma metabonomic profiling of lumbar disc - RSC Publishing
Aug 21, 2014 ... using gas chromatography-mass spectrometry (GC-MS) to profile metabolic characteristics of LDH and its ... placement of intervertebral disc with spinal nerve root com- ..... roots, since the roots normally adhere to surrounding tissues .... study of human lumbar discs has determined an increased level.
Download PDF - Plos
Jan 21, 2016 ... The intervertebral disc consists of the central nucleus pulposus (NP), which is encircled by the .... For immunohistochemical analysis human IVD tissue ... Endogenous peroxidase was blocked using 3% hydrogen peroxide in TBS for 1 hr and ... (UV)-spectrometry (Nanodrop, Thermo Scientific), and cDNA ...
Biomimetic Strategies to Engineer Mineralised Human Tissues
is overviewed the biomimetic strategies for bone tissue engineering, recent .... High-resolution magic angle spinning (HR-MAS) NMR spectroscopy enables to both ...... based hydrogels for intervertebral disc tissue-engineering applications.
JBC Papers in Press. Published on September 6, 2000 as
Sep 6, 2000 ... tissues such as the intervertebral disc (21), rib cartilage (22), and articular cartilage (11, 23). ... condyles within 18 hr after death from patients who had no clinical history of joint ... selected ion monitoring gas chromatography - mass spectrometry .... AGE levels in human cartilage collagen and skin collagen.
Covalent cross-linking of the NC1 domain of collagen type IX to
Nov 5, 2003 ... type IX collagen extracted by pepsin from fetal human cartilage, ... 8,9; avian cornea, 8), ear (tectorial membrane, 8), and intervertebral disc ... From both human and bovine tissues, cross-linked peptides ... for 24 hr at room temperature . .... analyzed by in-line HPLC/ion-trap mass spectrometry (LC/MS) after ...
1 DIFFERENCES IN CHAIN USAGE AND CROSS-LINKING
Dec 22, 2008 ... intervertebral disc (in which the bulk collagen monomer ... molecules is present in disc tissue compared with articular ... After 1 hr at 25˚C, the reaction .... mass spectrometry. .... terminus of human α1(XI) and α2(XI) chains were.
Novel corrective equations for complete estimation of human tissue
May 9, 2007 ... 1H-NMR spectroscopy of tissue homogenates has been shown to ... the spine, and prolapsed intervertebral disc, some torn fibres of the ..... P, Liacer JL, Piotto M , Assemat O, Celda B. 1H and 13C HR-MAS spectroscopy of ...
Novel corrective equations for.pdf
The isolation and characterization of magnesium whitlockite crystals
A recent study has reported the presence of such crystals in normal human articular ... Reports of 'cuboid' crystal deposition to date all relate to tissue that has been ... higher speeds to spin down particles of increasingly smaller mass. ..... 10.33 Whitlockite from intervertebral disc  10.31 Whitlockite from dental calculi  ...
pdf (1 MB)
cells may be more suitable for studying human development and tissue for- mation under diverse ..... gous MSCs in degenerated intervertebral discs. Patients.
Aug 2016 - Clemson University
Aug 4, 2016 ... Chief Human Resources Officer ...... Dissertation: Nutrient Related Mechanisms of Intervertebral Disc Degeneration .... Chemical Sensing Through Tissue .... Spectroscopy and Carbon Stable Isotope Ratio Mass Spectrometry.
Prescribing Information - Contrave
weight management in adults with an initial body mass index (BMI) of: ...... Musculoskeletal and Connective Tissue Disorders: intervertebral disc protrusion, .... Confirmatory tests, such as gas chromatography/mass spectrometry, will ... human dose [MRHD] of the naltrexone component in CONTRAVE on a .... 1,607 ng∙hr/mL.
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