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Automated nucleic acid purification from ThinPrep(R) PAP samples
Automated nucleic acid purification from ThinPrep® PAP samples for CT/NG detection by real time PCR and HPV detection by a laboratory-developed test using ...

Review - Journal of Molecular Diagnostics, The
first DNA amplification-based test for detection of Chla- .... to samples collected in ThinPrep PreservCyt solution (Ho- logic Inc. ... pending on the platform and availability of automation. .... including nucleic acid purification steps in the sample.

Comparison of DNA extraction from cervical cells - [email protected]
Jan 1, 2005 ... ThinPrep is the trade name for ... extraction, with or without a further purification step.20,21 ... microwaving and automated DNA extraction tech-.

Maxwell® 16 Applications Guide - reacting
Automated Nucleic Acid Purification Solutions ... Maxwell® 16 LEV Blood DNA Purification Kit – Cat.# AS1290 a ... Used 1ml of ThinPrep® stabilization solution.

Maxwell® 16 MDx Instrument: Streamlining Nucleic Acid Extrac]on
Automated Nucleic Acid Extrac]on. ✓ Use your ... Consistently purify high quality nucleic acids reducing the .... d ThinPrep® collechon device protocol. ✓ Original  ...

Summary - FDA
Dec 11, 2012 ... The Xpert CT/NO Assay is an automated in vitro diagnostic test for qualitative detection ... nucleic acid amplification, and detection of the target sequences in simple or complex samples using .... Cytyc ThinPrep 2000 .... The claimed LoD for purified CT serovar D elementary bodies in vaginal swab matrix is.

Long-term stability of human genomic and HPV DNA stored in BD
May 15, 2013 ... recovery of nucleic acid from SurePath™ and PreservCyt® media and inferred. 37 ... purification and subsequent downstream nucleic acid biochemistry such as PCR ..... Hologic, Inc. The ThinPrep® 2000 System Instructions for ... automated recovery of amplifiable DNA from tissues preserved in buffered.

PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a
nucleic acid amplification method to detect C. trachomatis in first-void urine specimens and ... mine whether the automated COBAS Amplicor CT/NG test can detect C. trachomatis ..... purified infectious elementary bodies of C. trachomatis ( sero-.

Performance Evaluation of Manual and Automated (MagNA Pure
(MagNA Pure) Nucleic Acid Isolation in HPV Detection and. Genotyping Using .... DNA form ThinPrep Pap tests using the linear array (LA). HPV genotyping and ... After nucleic acid purification all samples were analyzed by LA HPV assay for.

Roche Receives FDA Approval for Use of - Roche Diagnostics
Jul 7, 2016 ... States approved for both the ThinPrep and SurePath collection media”, said ... automation of nucleic acid purification, PCR (polymerase chain ...

PDF (897 KB) - Archives of Pathology & Laboratory Medicine
Sep 2, 2016 ... possibility of testing of higher-quality nucleic acids.7 In addition, fine-needle .... which is used to make both the cell block and a ThinPrep. (Hologic, Bedford ... that is directly compatible with our automated DNA extraction .... is attained are any sample purification steps performed. As mineral oil is an inert ...

PDF (246 KB) - Archives of Pathology & Laboratory Medicine
human papillomavirus DNA using the Roche cobas human ... excellent specificity with either ThinPrep or SurePath ... assay is based on 2 major processes: (1) automated ... b-globin DNA serving as process control, were purified through.

tech - Amazon Web Services
Sep 24, 2016 ... Pap (ThinPrep test), plasma, serum, swab. (cervical ... preparation, nucleic acid amplification, .... Fully automated nucleic acid purification.

Performance Evaluation of Manual and Automated (MagNA Pure
Apr 20, 2011 ... ... of Manual and Automated. (MagNA Pure) Nucleic Acid Isolation in HPV Detection and .... DNA form ThinPrep Pap tests using the linear array (LA) ... nucleic acid purification all samples were analyzed by LA HPV assay for.

Molecular Pathology/Probes - American Association for Clinical
Jul 30, 2014 ... Results: PCR amplification of DNA was detected using QIAxcel DNA Screening. Kit (Qiagen ... in DNA analyser: A case of laboratory automation .... After purification, the samples were again quantified to measure the ..... RNA prepared from ThinPrep® Pap (Hologic Inc., Bedford, MA, USA) samples, and the.

original version -
Oct 2, 2014 ... A commercial HPV DNA detection kit was first introduced to .... Using a nested PCR amplification for detection followed by automated DNA sequencing for .... ( ThinPrep System; Cytyc, Marlborough, MA, USA; or Surepath, BD ..... was used for HPV DNA PCR amplification without further purification. 3.2.

Download as a PDF - CiteSeerX
Human Papillomavirus (HPV) DNA in PreservCyt Samples by the Roche AMPLICOR and .... were rinsed into ThinPrep vials containing PreservCyt transport medium prior to ..... other automated nucleic acid purification platforms may pro-.

A Comparison of Two Methods to Determine the Presence of High
Oct 2, 2008 ... DNA, is the most widely used method to detect HPV infection in ... Cervical cytologic examination was performed using the automated ... The Invader-based method requires the use of purified extracted DNA. ..... reagents following automated extraction of DNA from cervical brushings in ThinPrep media.

Cervista HPV HR - Hologic
ThinPrep® Pap Test PreservCyt® Solution .... HR test allows simultaneous detection of HPV DNA sequences and HIST2H2BE in a single well .... AUTOMATED TEST PROCEDURE FOR THE CERVISTA® HTA SYSTEM ...... Purified amplicons.

Molecular tests for human papillomavirus (HPV), Chlamydia
Using the automated Sanger DNA sequencing method for parallel ... liquid-based cervicovaginal Pap cytology specimens (ThinPrep or Surepath) routinely ... of HPV, C trachomatis or N gonorrhoeae DNA without DNA extraction or purification , ...

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